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KMID : 0624620090420010041
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BMB Reports
2009 Volume.42 No. 1 p.41 ~ p.46
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Flavonoids inhibit the AU-rich element binding of HuC
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Kwak Ho-Joong
Jeong Kyung-Chae
Chae Min-Ju
Kim Soo-Youl
Park Woong-Yang
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Abstract
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Post-transcriptional regulation of mRNA stability by Hu proteins is an important mechanism for tumorigenesis. We focused on the molecular interactions between the HuC protein and AU-rich elements (AREs) to find chemical inhibitors of RNA-protein interactions using RNA electrophoretic mobility shift assay with non-radioactive probes. Screening of 52 natural compounds identified 14 candidate compounds that displayed potent inhibitory activity. Six (quercetin, myricetin, (-)-epigallocatechin gallate, ellagic acid, (-)-epicatechin gallate, and rhamnetin) were categorized as phytochemicals, and their IC50 values were low (0.2-1.8 μM).
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KEYWORD
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AU-rich element, Electrophoretic mobility shift assay, Flavonoids, HuC, Natural compounds
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